We are seeking a candidate with a strong background in cell physiology and molecular biology with a diploma or masters degree in biology/physiology/cell biology for a PhD-thesis to work on patho-physiological mechanisms of hypoxia- and inflammation-induced pulmonary edema will be investigated.
Methods: Functional and molecular studies will be performed on a co-culture system of primary alveolar epithelial cells with lung micro-vascular endothelial cells (in vitro model of the alveolar barrier). Inflammatory processes will be initiated by adding stimulated alveolar macrophages. Rat lung barrier function will also be studied in vivo.
Signaling mechanisms will be studied by gene silencing/overexpression and functional measurements.
Time: About 3 to 4 years
Begin: January 2010
Achievements: The results of this study will help understand molecular mechanisms that cause lung epithelial and endothelial damage and edema formation in inflammation and hypoxia. These are involved in immune-defense mechanisms such as in pneumonia, ARDS, ALI. Molecular studies will help clarify involved signalling pathways.
Medical Clinic VII, Sports Medicine
INF 410, Heidelberg, Germany